Figure 5
Expression of AKAP12 potentiates activation of Erk1/2 by beta-adrenergic agonist in A431 cells. A, time course of ERK phosphorylation in A431 cells stimulated with 10 μM isoproterenol. B, overexpression of HA-AKAP12 potentiates ERK activation in A431 cells stimulated with 10 μM isoproterenol. C, immunoblot analysis of expression of HA-AKAP12. The blots shown are taken from a single experiment, representative of at least three separate experiments performed on separate cultures of cells. Tabular data from separate experiments was analyzed for variance using the Student's "t" test for significance. * p < 0.05.