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Figure 4 | Journal of Molecular Signaling

Figure 4

From: AKAP12 and AKAP5 form higher-order hetero-oligomers

Figure 4

Expression of AKAP12 potentiates Erk1/2 activation by beta-adrenergic agonist in HEK293 cells. The cells were transiently transfected with HA-AKAP12 for 42 h and then changed to serum-free medium for 4 h. A, time course of ERK phosphorylation in HEK293 cells stimulated with 10 μM isoproterenol. B, overexpression of HA-AKAP12 potentiates ERK activation in HEK293 cells stimulated in response to 10 μM isoproterenol. C, Immunoblot analysis of expression of HA-AKAP12 using β-catenin as loading index. The blots shown are taken from a single experiment, representative of at least three separate experiments performed on separate cultures of cells. Tabular data from separate experiments was analyzed for variance using the Student's "t" test for significance. * p < 0.05.

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