Figure 4
Tumors derived from high Rad6B expressors show homogeneous EMT phenotype that is suppressed by mutant LRP6. (A) Tumor masses produced by the indicated MDA-MB-231 subpopulations. (B) Tumor morphologies of xenografts derived from the indicated MDA-MB-231 subpopulations by H&E staining. (C) Immunohistochemical analysis of Rad6, β-catenin, Vimentin and Snail1 in vector control, R6B-Zshigh, R6B-Zshigh/LRP6Δ173 and R6B-Zslow tumors. Original magnification ×20. (D) TUNEL staining (green) of tumors produced by the indicated MDA-MB-231 subpopulations. Sections were counterstained with propidium iodide. Original magnification ×40.