Figure 5
Oligomerization of AKAP5 expressed and purified from E. coli. His-tagged AKAP5 was expressed in E. coli. Affinity purified AKAP5 was resolved on either SDS-PAGE (A, C) or SDS-PAGE performed in the presence of 8 M urea (B, D). The gels were fixed and stained for protein with Coomassie Brilliant Blue (A, B) or the resolved proteins transferred to PVDF membrane and subjected to immunoblotting (C, D). Immunoblotting was performed using anti-AKAP5 antibody (C, D). The "◀" indicates the observed M r on the gels and the "Δ" indicates the predicted M r , calculated from the MW (47 kDa).