Table 1 Primers used for PCR
Transcript (Primer Name) | Forward Primer | Reverse Primer | Product Size |
|---|---|---|---|
H. E4BP4 (HUMANE4BP4-742 & 991) | 5'ATGGGGAATTCTTTCTCTGG3' | 5'CTTTGATCCGGAGCTTGTGT3' | 250 bp |
M. E4BP4 (Mouse E4BP4-742 &1117) | 5'ATGGGAAGCTCTTTCTCCACT3' | 5'TACCCGAGGTTCCATGTTTC3' | 376 bp |
Bim (BIM 5/6 SENSE & ANTI) | 5'CAGATATGCGCCCAGAGATA3' | 5'ACCAGGCGGACAATGTAAC3' | 163 bp |
Puma (PUMA SENSE & ANTI) | 5'AAGAGCAAATGAGCCAAACG3' | 5'GCAGAGCACAGGATTCACAG3' | 181 bp |
β-actin (B-ACTIN-130 SENSE & ANTI) | 5'AGTCCTCTCCCAAGTCCACA3' | 5'CACGAAGGCTCATCATTCAA3' | 130 bp |