Figure 5
RU38486 blocks Dex-mediated effects in CEM C1-15mE#3 cells : Panel A: CEM C1-15mE#3 cells were seeded at a density of 1 × 105cells/ml and treated for 72 h with either 0.1% ethanol (EtOH), 1 μM Dex, 1 μM RU486, or both Dex and RU486. Trypan blue excluding viable cells were counted at 24h intervals. Data represent averages ± SD from triplicate sets of treatments from two experiments. Panel B: CEM C1-15mE#3 cells were treated as in Panel A for 24h and RNA was extracted with TriZol. RNA was subjected to reverse transcription reaction and real-time qPCR using primers specific for Bim or Puma as described for Figure 4. Fold change in expression was calculated by the Pfaffl method using β-actin as a reference. Data represent averages ± SD from two independent experiments run in duplicates.