Figure 3

Flow cytometric cell cycle analysis demonstrates sub-G1 accumulation of CEM C1-15mE#3 cells treated with Dex: CEM C7-14, CEM C1-15 and CEM C1-15mE#3 cells were treated with 0.1% ethanol (EtOH) or 1 μM Dex for 24, 48 and 72 h, stained in propidium iodide, and analyzed for cell cycle distribution flow cytometrically using an Accuri C6 flow cytometer and CFlow^® software. Panel A: Representative histograms of cell cycle distribution of ethanol and Dex treated cells after 72 h treatment. For each analysis 50,000 singlet cells were gated into sub-G1, G1, S and G2/M as indicated for the top left histogram. Panel B: Time course of accumulation of cells with sub-G1 DNA content as a percentage of total cells analyzed. Average ± S.D. from three independent experiments. Panel C: Data from three independent experiments were subjected to a paired Student's T-test (two sample, equal variance). Gray shaded boxes represent significant differences with p-values < 0.05.