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Figure 8 | Journal of Molecular Signaling

Figure 8

From: Canonical Wnt signaling is involved in switching from cell proliferation to myogenic differentiation of mouse myoblast cells

Figure 8

FH535 and K252a alter total and phospho-β-catenin (Y654) in C2C12 cells. (A) Western blots of cytosolic and membrane fractions from C2C12 cells treated with FH535 and K-252a using antibodies against total cellular-β-catenin (upper panel) and phosphorylated β-catenin (lower panel). GRP78 indicates loading control. (B, C) Relative levels of total and phosphorylated β-catenin in cytosolic (black) and membrane (grey) fractions were calculated as the β-catenin/GRP78 ratio. Band intensities are relative to the control cell values in the cytosolic fraction and defined as 1.0. Similar results were obtained in three independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187