Figure 2From: Canonical Wnt signaling is involved in switching from cell proliferation to myogenic differentiation of mouse myoblast cells Wnt4 promotes myogenic differentiation of C2C12 cells. Expression constructs of Wnt family members in adenovirus vector were transfected into C2C12 cells and myogenic differentiation was determined by troponin T immunostaining after 2 days culture in differentiation medium, which commenced 24 h post-transfection.Back to article page