Figure 1From: Canonical Wnt signaling is involved in switching from cell proliferation to myogenic differentiation of mouse myoblast cells Endogenous Wnt signaling component expression during myogenic differentiation of C2C12 cells. Wnt signaling molecule expression was measured by quantitative RT-PCR. After 2 or 4 days culture in differentiation medium, cells were harvested, total RNA prepared and relative gene expression quantified by real-time PCR as follows: (A) wingless-related MMTV integration, Wnt; secreted frizzled-related protein, Sfrp; and porcupine, Porcn. (B) frizzled, Fzd; and low density lipoprotein receptor-related protein, Lrp. (C) cyclin D, Ccnd; myelocytomatosis oncogene, Myc; fos-like antigen 1, Fosl1; casein kinase 1, alpha 1, Csnk1a1; catenin, beta 1, Ctnnb1; dishevelled 1, Dvl1; glycogen synthase kinase 3 beta, Gsk3b; transcription factor 3, Tcf3; and transducin-like enhancer of split 2, Tle2. Day 2 and 4 time points were normalized to expression levels at day 0. Data are from three independent experiments, *P < 0.005 and † P < 0.05.Back to article page