Figure 2

Substrate surface spreading of P-Rex1-/- platelets. Washed mouse platelets from wild type (P-Rex1 ^+/+) or P-Rex1 ^-/- mice were placed on 100 μg/ml fibrinogen-coated (A), 100 μg/ml fibrillar collagen-coated (B) or 50 μg/ml thrombin-coated (C) coverslips in the presence of vehicle, 2 U/ml apyrase (+apy), apyrase and 1 U/ml thrombin (+apy/+thr) or 10 μM ADP for 45 min at 37°C and imaged by DIC microscopy. The individual surface areas of 300 P-Rex1 ^+/+ (black line) and 300 P-Rex1 ^-/- (grey line) platelets were quantified using Image J software and plotted as a frequency distribution. DIC images and platelet surface area histograms are representative of > 3 experiments. Scale bar = 10 μm.