5 MDa of the Dvl3-based signalsome. Fluorescence correlation spectroscopy (fcs) is a single molecule analysis performed in live cells that experimentally measures the diffusion coefficient and permits calculation of MW of the signalsome (0.2 and 30 MDa species in vivo), which also reveal an upfield shift in MW in response to Wnt3a. Proteomics provides for molecular dissection of the composition of the signalsome isolated from untreated and Wnt3a-treated cells. Conclusion Dvl3-based punctae/signalsomes made visible by fluorescent microscopy now can be interrogated by advanced physical means, defining such properties as signalsome M r /MW, molecular composition, and intracellular locale."/>
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Figure 2 | Journal of Molecular Signaling

Figure 2

From: Probing the physical nature and composition of signalsomes

Figure 2

Fluorescence correlation spectroscopy enables measurements of diffusion constants and calculate MW of fluorescently-tagged molecules core to supermolecular complexes of interest, such as the Dvl3-based signalsomes. Fcs is a single molecule analysis of the dwell time of a molecule/complex of interest, sampled in situ in live cells. Please consult the Methods section for a detailed explanation of the theory and practical aspects of applying fcs measurements in live cells.

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