Figure 2
From: Probing the physical nature and composition of signalsomes
Fluorescence correlation spectroscopy enables measurements of diffusion constants and calculate MW of fluorescently-tagged molecules core to supermolecular complexes of interest, such as the Dvl3-based signalsomes. Fcs is a single molecule analysis of the dwell time of a molecule/complex of interest, sampled in situ in live cells. Please consult the Methods section for a detailed explanation of the theory and practical aspects of applying fcs measurements in live cells.