5 MDa of the Dvl3-based signalsome. Fluorescence correlation spectroscopy (fcs) is a single molecule analysis performed in live cells that experimentally measures the diffusion coefficient and permits calculation of MW of the signalsome (0.2 and 30 MDa species in vivo), which also reveal an upfield shift in MW in response to Wnt3a. Proteomics provides for molecular dissection of the composition of the signalsome isolated from untreated and Wnt3a-treated cells. Conclusion Dvl3-based punctae/signalsomes made visible by fluorescent microscopy now can be interrogated by advanced physical means, defining such properties as signalsome M r /MW, molecular composition, and intracellular locale."/>
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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Probing the physical nature and composition of signalsomes

Figure 1

Steric-exclusion chromatography enables isolation and molecular sieving of large, supermolecular complexes/signalsomes. One configuration of steric-exclusion chromatography optimized in the authors' laboratory makes use of the Pharmacia AKTA-based liquid handler equipped with large diameter, long (30-60 cm) columns packed with wide-bore matrices such as Sephacryl 200, 400, or larger. The system can accommodate large sample loading and provides excellent performance in efforts designed to isolated very large supermolecular complexes like the Dishevelled3-based signalsomes highlighted in this article.

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