Figure 1

Steric-exclusion chromatography enables isolation and molecular sieving of large, supermolecular complexes/signalsomes. One configuration of steric-exclusion chromatography optimized in the authors' laboratory makes use of the Pharmacia AKTA-based liquid handler equipped with large diameter, long (30-60 cm) columns packed with wide-bore matrices such as Sephacryl 200, 400, or larger. The system can accommodate large sample loading and provides excellent performance in efforts designed to isolated very large supermolecular complexes like the Dishevelled3-based signalsomes highlighted in this article.