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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Glycogen Synthase Kinase-3beta regulates Snail and beta-catenin during gastrin-induced migration of gastric cancer cells

Figure 6

Effect of modulation of GSK3β, β-catenin and Snail pathways on G17-induced MMP7 transcription. (A). Subconfluent AGSE cells were co-transfected with MMP7-luciferase and β-Gal vectors along with either EV (lanes 1, 2), GSK3β-S9A (lanes 3, 4) or GSK3β-K/A (lanes 5, 6) mutants. Luciferase and β-Gal assays were performed after G17 treatment as described under 2D. (B) AGSE cells were transfected as in A along with either control-siRNA (lanes 1, 2), β-catenin-siRNA (lanes 3, 4), Snail-siRNA (lanes 5, 6) or a combination of β-catenin and Snail-siRNA (lanes 7, 8). G17 treatment was performed as described under 2D, followed by luciferase and β-Gal assays. Each transfection (A and B) was performed in triplicate, and the data represent the mean ± SD of at least two independent experiments.

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