Figure 3
From: EGFR/Met association regulates EGFR TKI resistance in breast cancer
HGF is not expressed in SUM229 breast cancer cells. (A) Lysates from SUM149 and SUM229 and conditioned media from RMF-HGF cells were immunoblotted with anti-HGFα. Recombinant HGF was used as a positive control. (B) Conditioned media was collected from confluent SUM149, SUM229, and RMF-HGF cell cultures. Diluted conditioned media was analyzed for HGF expression using ELISA with RMF-HGF as a positive control. The amount of HGF produced from the SUM149 and SUM229 lysates was undetectable.