Figure 1

SSBP2 is localized in the nucleus of HEK293 cells in response to CRM1-export pathway inhibition. Exponentially growing HEK293 cells were treated with vehicle (upper panel) or 10nM LMB (lower panel) for 3 hrs. prior to fixation and staining with antibodies to SSBP2 [7] or PML (red). Cells were co-stained with DAPI and the images obtained by deconvolution algorithms are shown. In control cells SSBP2 localizes to the large aggresome, while PML is in distinct nuclear bodies (upper panel). When nuclear export is blocked by LMB treatment, SSBP2 and PML localize to nuclear structures (lower panel).