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Figure 2 | Journal of Molecular Signaling

Figure 2

From: AKAR2-AKAP12 fusion protein "biosenses" dynamic phosphorylation and localization of a GPCR-based scaffold

Figure 2

Imaging of AKAR2 expressed in human epidermoid carcinoma A431 cells. Panel A, images of AKAR2 biosensor when expressed in A431 cells. The fluorescence images were recorded at 473-495 nm for CFP, and, 527-591 nm for YFP (citrine). Panel B, AKAR2-based fluorescence images were recorded at 473-495 nm for CFP and 527-591 nm for YFP (citrine), in A431 cells in which the nuclei were stained with DAPI. The DAPI recordings were performed at 435-485 nm. The images shown are representative of a large array of images collected for this purpose.

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