MCP110 and MCP116 but not MCP146 inhibit the Ras/LET-60-induced Muv phenotype. A. Representative images of untreated wild-type animals (WT phenotype with normal vulva (arrowhead) but no ventral protrusions); animals harboring constitutively activated Ras treated with vehicle only (Muv phenotype; arrows mark ventral protrusions formed by pseudovulvae); the same strain following treatment with MCP110 (20 μM) (WT phenotype, as indicated by lack of ventral protrusions). B. Animals harboring constitutively activated Ras as in Panel A were treated with either vehicle (DMSO) or MCP110, MCP116, MCP146 or negative control MCP122 (3, 10 or 20 μM; higher concentrations precipitated out of solution). The Y-axis indicates the percentage of MCP-treated animals with a Muv phenotype, normalized to vehicle-treated animals. Data were analyzed by three-way ANOVA. (***) and (*) indicate p values of < 0.0001 and < 0.05, respectively.