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Figure 3 | Journal of Molecular Signaling

Figure 3

From: TLN-4601 suppresses growth and induces apoptosis of pancreatic carcinoma cells through inhibition of Ras-ERK MAPK signaling

Figure 3

TLN-4601 inhibits the K-Ras downstream signaling cascade in PDAC cells. Exponentially growing cells were treated with increasing concentrations of TLN-4601. After overnight exposure, cells were lysed and cellular extracts (30 μg protein) were separated on a 10% SDS-PAGE gel and transferred to nitrocellulose membranes. (A) Membranes were sequentially probed with Raf-1 and ERK1/2 (p44/42 MAP kinase) or (B) phospho-MEK1/2 (Ser217/221), MEK1 and vinculin (loading control). Blots are representatives of three independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187