Figure 1

Cell survival of H ₂ O ₂ -treated PC12-GFP and PC12-SH2B1β cells. PC12-GFP cells (A, C, E) and PC12-SH2B1β cells (B, D, F) were treated with 0 (A, B), 50 (C, D), or 100 (E, F) μM H₂O₂ for 24 h. Representative images of live cells were shown. (G) Parental PC12 cells were treated with 100 μM H₂O₂ for the indicated time points. ROS levels were measured using DHE dye as described in "Materials and Methods". Arrows point to dying cells. Arrowheads point to live cells.