Immature receptors dictate dimers interaction with chaperones. HEK293 cells were transfected with AT1R (WT or ND)-v1/v2 and β2AR (WT or NQ)-v1/v2. After 48 hours, cells were harvested, washed, lysed with RIPA and precleared with protein A-sepharose beads. The lysate was distributed into eight different microcentrifuge tubes and co-immunoprecipitations were performed using the indicated chaperone antibody. The eighth sample was loaded as a control to show the expression level of the different receptor dimers as shown in g. Results are representative of 4 independent experiments.