Interaction of wild type receptor dimers with chaperones. HEK293 cells were transfected with AT1R (WT)-v1/v2 and β2AR (WT)-v1/v2. After 48 hours, cells were harvested, washed, lysed with RIPA. This lysate was distributed into eight different microcentrifuge tubes and co-immunoprecipitations were performed using the indicated chaperone antibody. a) AT1R/β2AR (WT) heterodimer, b) AT1R (WT) homodimer c) β2AR (WT) homodimer. The eighth sample was loaded as a control to show the expression level of the wild type receptor dimers as shown in d. Results are representative of 4 independent experiments.