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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Chaperones contribute to G protein coupled receptor oligomerization, but do not participate in assembly of the G protein with the receptor signaling complex

Figure 1

Expression of the receptor dimer pairs. a) Schematic presentation of the constructs used in the experiments. Venus1 contains the N- terminal end of the yellow fluorescent protein variant (venus) and represents the first 157 amino acids while venus2 contains the C- terminal end of the protein and represents amino acids 158 to 238. b) HEK293 cells were harvested, washed and lysed with RIPA buffer. Samples were then run on SDS-PAGE and Western analysis was performed using indicated chaperone antibodies (1:2000). c) Specificity of GFP antibody to dimers was tested in HEK293 cells. The cells were transfected with AT1R-v1 or v2, β2AR-v1 or v2 and AT1R-v2 with β2AR-v1. 48 hours after transfection, cells were lysed in RIPA buffer. Western analysis was performed using anti-GFP (1:2000) and indicate the presence of a yellow fluorescent protein variant, venus. Results are representative of 3 independent experiments.

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