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Figure 6 | Journal of Molecular Signaling

Figure 6

From: The dietary bioflavonoid quercetin synergizes with epigallocathechin gallate (EGCG) to inhibit prostate cancer stem cell characteristics, invasion, migration and epithelial-mesenchymal transition

Figure 6

Regulation of epithelial mesenchymal transition factors by EGCG in prostate cancer stem cells isolated from primary tumors. (A), Prostate CSCs were treated with EGCG (0-60 μM) for 48 h. At the end of incubation period, the expression of vimentin, slug, and snail was measured by the Western blot analysis. (B), Effects of EGCG on the expression of nuclear β-catenin. Prostate CSCs were treated with EGCG (0-60 μM) for 48 h. At the end of incubation period, cells were harvested and nuclear fractions were prepared. The expression of β-catenin and GAPDH was measured by was measured by the Western blot analysis. (c), Effects of EGCG on TCF-1/LEF activity. Prostate CSCs were transduced with lentiviral Top-dGFP-reporter (pRLR.sm-18.ppt). Transduced CSCs were treated with EGCG (0-60) for 3 days and the GFP fluorescence was measured. Data represent mean ± SD. * or ** = significantly different from control, P < 0.05.

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Journal of Molecular Signaling

ISSN: 1750-2187