Figure 2

Expression patterns of CrDHS1 in response to UV-B irradiation in C. roseus cultured cells. Six-day-old cultured cells were irradiated for 5 min with UV-B light or left untreated, harvested at the indicated time points, and total RNA was isolated and used for RT-PCR as described in materials and methods. Equal cDNA amounts were ensured by amplification of constitutively expressed Rps9 gene as positive control. Quantification of CrDHS1 and CrRPS9 expression was performed. The mean ± S.D. values of three independent experiments using Image Guage software (version 2.54) are shown in an inset. Each figure shows visual (gel plots) and graphical representation of the expression