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Figure 4 | Journal of Molecular Signaling

Figure 4

From: Transformation by a nucleotide-activated P2Y receptor is mediated by activation of Gαi, Gαq and Rho-dependent signaling pathways

Figure 4

Expression of tP2YR results in lamellipodia formation and activation of both Rac1 and RhoA. NIH3T3 cells were transiently transfected with either empty pcDNA3 expression vector or those encoding tP2YR, Tiam1-C1199 (A, potent activator of Rac1) or Ect2-DH/PH/C (B, potent activator of RhoA). Activated GTP-bound Rac1 and RhoA were isolated in pull-down assays with GST-Pak-PBD and GST-Rhotekin-RBD, respectively.

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Journal of Molecular Signaling

ISSN: 1750-2187