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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Transformation by a nucleotide-activated P2Y receptor is mediated by activation of Gαi, Gαq and Rho-dependent signaling pathways

Figure 3

tP2yR synergizes with activated Raf1 to induce foci with similar characteristics to activated Rac1. A. Quantitation of the number of foci induced when tP2YR or activated Rac1(115I) were co-transfected with activated Raf-1(340D). NIH3T3 cells were co-transfected with 1 μg of pZIP-NeoSV(x)1 retrovirus expression plasmids encoding tP2YR or Rac1(115I) and either 1 μg of the empty pZIP-NeoSV(x)1 plasmid DNA (-) or encoding the weakly activated Raf-1(340D) mutant (+). The appearance of foci of transformed cells was quantitated after 14 days. Data shown are the mean ± standard error for triplicate plates and are representative of three independent assays. B. The appearance of transformed foci induced in NIH3T3 cells were co-transfected with activated Raf-1(340D) is similar to those induced by activated Rac(115I) and distinct from those induced by activated Rho(63L). pZIP-NeoSV(x)1 expression vectors encoding RhoA(63L), G2A, Mas, Rac1(115I) or tP2YR were co-transfected with pZIP-raf-1(340D). pZIP-H-ras(61L) was co-transfected with the empty pZIP-NeoSV(x)1 plasmid DNA. The appearance of foci of transformed cells was monitored 14 days after transfection (40× magnification).

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Journal of Molecular Signaling

ISSN: 1750-2187