Figure 5

Akt Activation by AICAR is suppressed by Wortmannin. A. 3T3-F44a preadipocytes were incubated with Wortminnin (Wort, 0.5 μM, 30 min), followed by AICAR (1 mM, 4 h) or insulin (100 nM, 15 min). The cell extracts were blotted with antibodies, as indicated. B. 3T3-F44a preadipocytes were treated with Wortmannin at different doses, as indicated, for 30 min and followed by treatment with AICAR or insulin, as described for A. Cell extracts were blotted with antibodies, as indicated.