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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Romidepsin inhibits Ras-dependent growth transformation of NIH 3T3 fibroblasts and RIE-1 epithelial cells independently of Ras signaling inhibition

Figure 6

Romidepsin does not inhibit Ras-mediated ERK or AKT activation in NIH 3T3 and RIE-1 cells. NIH 3T3 (A) or RIE-1 (B) cells stably expressing the indicated proteins were treated with DMSO (Vehicle), 5 nM romidepsin, 10 μM U0126, or 10 μM LY294002 for 24 h. Cell lysates were analyzed by western blotting with antibodies to total ERK1/2, phospho-ERK1/2, total AKT, phospho-Akt, B-Raf, pan-Ras, and vinculin (loading control). Data shown are representative at least two independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187