Skip to main content
Figure 4 | Journal of Molecular Signaling

Figure 4

From: Romidepsin inhibits Ras-dependent growth transformation of NIH 3T3 fibroblasts and RIE-1 epithelial cells independently of Ras signaling inhibition

Figure 4

Romidepsin inhibits anchorage-independent growth of transformed NIH 3T3 and RIE-1 cells. NIH 3T3 cells (A) and RIE-1 cells (B) stably expressing the indicated proteins were evaluated for colony formation in soft agar in complete growth medium supplemented with the indicated concentrations of romidepsin. Following 23 d (NIH 3T3) or 15 d (RIE-1), viable colonies were stained in MTT and plates were scanned. The number of colonies was quantified from the scanned images using ImageJ software. Data shown are the average ± SD of triplicate plates and are representative of two independent experiments.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187