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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Romidepsin inhibits Ras-dependent growth transformation of NIH 3T3 fibroblasts and RIE-1 epithelial cells independently of Ras signaling inhibition

Figure 1

Romidepsin inhibits the anchorage-dependent growth of Ras-transformed NIH 3T3 and RIE-1 cells. NIH 3T3 cells (A) and RIE-1 cells (B) stably-infected with the empty pBabe-puro vector or encoding constitutively active human H-Ras(G12V), K-Ras(G12V), N-Ras(G12D), or B-Raf(V600E), or rat ErbB2/Neu(V664E) were seeded on day 0. On day 1, cells were treated with complete growth medium supplemented with DMSO (Vehicle), or 1, 3, or 5 nM romidepsin. After 72 h of romidepin treatment (day 3), cell viability was measured using the MTT assay. Data shown are the average ± SD of eight replicate wells and are representative of three independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187