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Figure 6 | Journal of Molecular Signaling

Figure 6

From: AKT/eNOS signaling module functions as a potential feedback loop in the growth hormone signaling pathway

Figure 6

Phosphorylation states of CDC2 in a time course of GH treatment in the cell culture with or without AKT inhibitor (AKTi) pre-treatment. After treatment with GH at the indicated time points, proteins were extracted from MDBK cells using the ProteoExtract® subcellular extraction kit (Calbiochem). Two identical gels were transferred to nitrocellulose membrane and Western blot analyses were performed for total CDC2/Cdk1 and phosphor-CDC2/Cdk1. Panel A: Temporal changes of phospho-CDC2/Cdk1 (p-(Y15)-CDC2/Cdk1 and total CDC2/Cdk1 after administration of GH. Panel B: Temporal changes of phosphor-CDC2/Cdk1 (p-(Y15)-CDC2/Ckd1) and total CDC2/Cdk1 in AKT inhibitor (AKTi)-pretreated MDBK cells after administration of GH. Panel C: Quantitation of phosphor-CDC2/Cdk1 (p-(Y15)-CDC2/Cdk1) from panels A and B. n = 2 replicated experiments.

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