Panel A: KPSS-6.0 phosphoantibody multi-immunoblot array; MDBK cells were treated with or without the AKT inhibitor for 30 minutes before GH administration. The whole cell lysates were prepared after 30 min of GH challenge and analyzed using the KPSS-6.0 phosphoantibody multi-immunoblot array by Kinexus http://www.kinexus.ca/KPSS.htm. The phosphor-protein targets of interest are indicated by arrows and abbreviations. The upper panel gives the array analysis of normal cells without GH (control); the middle panel shows the array analysis of GH treated cells; the lower panel gives the array analysis of the AKT inhibitor pretreated cells before GH administration. Panel B: Normalized quantitation of the results of KPSS-6.0 phosphoantibody multi-immunoblot array. The quantitation is the trace quantity of the band corrected to a scan time of 60 seconds. The normalized quantitation is the quantitation normalized to correct for differences in protein amounts.