Figure 4From: AKT/eNOS signaling module functions as a potential feedback loop in the growth hormone signaling pathway Both AKT and eNOS inhibitors eliminate the nitration of JAK2. GH was administrated to cell cultures with or without either AKT or eNOS inhibitor pre-treatment for 30 min. Whole cell extracts were analyzed with Western blot using an antibody (Anti-nitro-1007-Y1008Y-JAK2). AKTi: AKT inhibitor, eNOSi: eNOS inhibitor. Loading control: Identical gel stained with SimplyBlueâ„¢ (Invitrogen).Back to article page