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Figure 4 | Journal of Molecular Signaling

Figure 4

From: AKT/eNOS signaling module functions as a potential feedback loop in the growth hormone signaling pathway

Figure 4

Both AKT and eNOS inhibitors eliminate the nitration of JAK2. GH was administrated to cell cultures with or without either AKT or eNOS inhibitor pre-treatment for 30 min. Whole cell extracts were analyzed with Western blot using an antibody (Anti-nitro-1007-Y1008Y-JAK2). AKTi: AKT inhibitor, eNOSi: eNOS inhibitor. Loading control: Identical gel stained with SimplyBlueâ„¢ (Invitrogen).

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