Both AKT and eNOS inhibitors eliminate the nitration of JAK2. GH was administrated to cell cultures with or without either AKT or eNOS inhibitor pre-treatment for 30 min. Whole cell extracts were analyzed with Western blot using an antibody (Anti-nitro-1007-Y1008Y-JAK2). AKTi: AKT inhibitor, eNOSi: eNOS inhibitor. Loading control: Identical gel stained with SimplyBlue™ (Invitrogen).