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Figure 1 | Journal of Molecular Signaling

Figure 1

From: AKT/eNOS signaling module functions as a potential feedback loop in the growth hormone signaling pathway

Figure 1

Panel A: Cells with or without pre-treatment of AKT inhibitor (AKTi) were challenged with GH at 200 ng/ml for 0, 15 and 30 min respectively. Cellular proteins were extracted from MDBK cells using the ProteoExtract® subcellular extraction kit (Calbiochem) following the manufacturer's instructions. Western blotting experiments were conducted and analyzed as specified in Material and Methods. Panel B shows the quantitation of AKT phosphorylation (p-Thr308) after GH treatment.

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