Figure 4

Medium alkalinization of C. roseus suspension cultured cells in response to UV-B irradiation. Four- and six-day-old cell suspension cultures were irradiated with UV-B for 5 min and the pH of the medium was measured at indicated times after the start of irradiation. Alkalinization Response (AR) or ΔpH was measured as described in materials and methods. Experiments were repeated with two different populations of cells and each experiment was carried out in triplicates.