Figure 9
β-arrestin 2 K18R, K107R, K108R, K207R, K296R demonstrates impaired agonist-promoted co-localiztion with M 2 mAChR in MEF KO1/2. MEF KO1/2 cells were transfected with HA-M2 mAChRs (green) and either wild-type FLAG-β-arrestin 2, FLAG-β-arrestin 2K18R, K107R, K108R, K207R, K296R or FLAG-β-arrestin 2K11R, K12R (red). 24 hr after transfection, cells were treated with 1 mM carbachol for 30 min. Cells were fixed, probed, and imaged as described in methods. Wild-type and β-arrestin 2K11R, K12R demonstrate co-localization with the M2 mAChR which is significantly diminished with β-arrestin 2K18R, K107R, K108R, K207R, K296R. Image shown is representative of two independent experiments.