Figure 11
β-arrestin 2 K18R, K107R, K108R, K207R, K296R disrupts the co-localization of M 2 mAChR and LAMP-1 in MEF KO1/2. MEF KO1/2 cells were transfected with HA-M2 mAChR and either wild-type FLAG-β-arrestin 2, FLAG-β-arrestin 2K18R, K107R, K108R, K207R, K296R or FLAG-β-arrestin 2K11R, K12R. 24 hr after transfection, cells were treated with 1 mM carbachol for 6 hr. Cells were fixed, probed, and imaged as described in methods. β-arrestin 2K18R, K107R, K108R, K207R, K296R disrupts the co-localization of M2 mAChR and LAMP-1 compared to wild-type and β-arrestin 2K11R, K12R. Image shown is representative of three independent experiments.