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Figure 10 | Journal of Molecular Signaling

Figure 10

From: Differential role of beta-arrestin ubiquitination in agonist-promoted down-regulation of M1 vs M2 muscarinic acetylcholine receptors

Figure 10

β-arrestin 2 K18R, K107R, K108R, K207R, K296R demonstrates no co-localization with M 1 mAChR in MEF KO1/2. MEF KO1/2 cells were transfected with eGFP-M1 (A) mAChRs (green) and either wild-type FLAG-β-arrestin 2, FLAG-β-arrestin 2K18R, K107R, K108R, K207R, K296R or FLAG-β-arrestin 2K11R, K12R (red). 24 hr after transfection, cells were treated with 1 mM carbachol for 30 min. Cells were fixed, probed, and imaged as described in methods. None of the β-arrestin constructs demonstrate co-localization with the M1 mAChR. Image shown is representative of two independent experiments.

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