Skip to main content
Figure 2 | Journal of Molecular Signaling

Figure 2

From: G-protein-coupled receptor-associated A-kinase anchoring proteins AKAP5 and AKAP12: differential signaling to MAPK and GPCR recycling

Figure 2

Activation of the mitogen-activated protein kinase cascade to Erk1,2 in human epidermoid A431 cells in response to isoproterenol stimulation. Wild-type 431 or A431 cells treated with siRNAs targeting either AKAP12 or AKAP5, or both AKAPs, were stimulated by isoproterenol (10 μM) for different intervals. Cell lysates were subjected to SDS-polyacrylamide gel electrophoresis, the resolved proteins transferred to nitrocellulose. The blots were stained by antibody against activation-specific phospho-Erk1,2, against pan-Erk, or against AKAP12 and AKAP5.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187