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Figure 1 | Journal of Molecular Signaling

Figure 1

From: G-protein-coupled receptor-associated A-kinase anchoring proteins AKAP5 and AKAP12: differential signaling to MAPK and GPCR recycling

Figure 1

Overlay analysis of A kinase-anchoring proteins in A431 and HEK cells. Cell lysates (50 μg of protein/lane) of A431 or HEK cells were subjected to SDS-polyacrylamide gel electrophoresis, the resolved proteins transferred to nitrocellulose blots, and the blots subjected to renaturation and stained with A-kinase RII subunit for 2 h at room temperature. After washing, the presence of AKAPs was detected using an goat anti-RII antibody. The resultant blots also were stained with antibodies specific for AKAP12 or AKAP5.

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Journal of Molecular Signaling

ISSN: 1750-2187