Figure 1From: Cell cycle arrest in Metformin treated breast cancer cells involves activation of AMPK, downregulation of cyclin D1, and requires p27Kip1 or p21Cip1 Metformin inhibits proliferation of cultured breast cancer cells. A. MCF7 cells were treated with metformin at the indicated concentrations for one day and then cell number was determined using a hemocytometer. The mean cell number from 3 independent cultures is shown. Error bars represent standard deviation. Using a t test, all treatments were significantly different from the control with P values of less than 0.01. B. Six different breast cancer cell lines were treated with 8 mM metformin. At the indicated times viable cell number was determined using a hemocytometer. The reported status of estrogen receptor (ER) expression, HER2 amplification, and p53 expression (Wild-type, WT, or mutant, mt) are indicated for each cell line. HER2+ indicates HER2 amplification while HER2- indicates normal HER2 expression. The data points represent the mean cell number from 3 independent cultures and error bars represent standard deviation.Back to article page