Journal of Molecular Signaling

Table 5 Expression of Wnt gene in mouse totipotent teratocarcinoma embryonic cell (F9), human embryonic stem cell (H7), and human mesenchymal cells (hM).

From: Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells

gene\cell	F9	H7	hM
Wnt 1	+	+	-
Wnt 2	-	+	+
Wnt 2b	+	+	+
Wnt 3	+	+	+
Wnt 3a	+	+	+
Wnt 4	+	+	+
Wnt 5a	+	+	+
Wnt 5b	+	+	+
Wnt 6	+	+	+
Wnt 7a	+	+	+
Wnt 7b	+	+	+
Wnt 8a	+	+	-
Wnt 8b	+	+	-
Wnt 9a	+	+	+
Wnt 9b	+	+	-
Wnt 10a	+	+	-
Wnt 10b	+	+	+
Wnt 11	+	+	-
Wnt 16	+	+	+

RNA was extracted from F9, H7 and hM and reverse-transcribed to obtain first strand cDNA. Specific primers were used in PCR to amplify each Wnt cDNA. Amplified products were purified and confirmed by DNA sequencing.

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