Table 2 Primer sequences for RT-PCR analysis of mouse Frizzled (mFzd) and LRP genes
From: Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells
Gene | Accession No. | Sequence (5' to 3') | Amplicon (bp) |
|---|---|---|---|
mFz1 | NM_021457 | (F) caaggtttacgggctcatgt (R) tgaacagccggacaggaaaa | 178 |
mFz2 | NM_020510 | (F) ccgacggctctatgttcttc (R) tagcagccggacagaaagat | 173 |
mFz3 | NM_021458 | (F) tgggttggaagcaaaaagac (R) cctgctttgcttctttggtc | 239 |
mFz4 | NM_008055 | (F) gccaatgtgcacagagaaga (R) aggtggtggagatgaagcag | 398 |
mFz5 | NM_022721 | (F) ctgtggtctgtgctgtgctt (R) ggccatgccaaagaaataga | 264 |
mFz6 | NM_008056 | (F) tctgtgcctctgcgtatttg (R) tctcccaggtgatcctgttc | 218 |
mFz7 | NM_008057 | (F) gcttcctaggtgagcgtgac (R) aacccgacaggaagatgatg | 216 |
mFz8 | NM_008058 | (F) ttacatgcccaaccagttca (R)cggttgtagtccatgcacag | 306 |
mFz9 | NM_284144 | (F) agtttcctcctgaccggttt (R) ttttcggtagcacaggctct | 390 |
mFz10 | NM_175284 | (F) agattcccatgtgcaaggac (R) agttggggtcgttcttgttg | 321 |
mLRP5 | NM_008513 | (F) Aagaccctgcttgaggacaa (R) gagtgggatagccacatcgt | 402 |
mLRP6 | NM_008514 | (F) Gagctcatcggtgacatgaa (R) gctcgaggactgtcaaggtc | 400 |