Table 1 Primer sequences for RT-PCR analysis of mouse Wnt (mWnt) genes
From: Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells
Gene | Accession No. | Sequence (5' to 3') | Amplicon (bp) |
|---|---|---|---|
mWnt1 | NM_021279 | (F) ccgagaaacagcgttcatct (R) gcctcgttgttgtgaaggtt | 252 |
mWnt2 | NM_023653 | (F) Atctcttcagctggcgttgt (R) agccagcatgtcctcagagt | 326 |
mWnt2b | NM_009520 | (F) cacccggactgatcttgtct (R) tgtttctgcactccttgcac | 236 |
mWnt3 | NM_009521 | (F) cgctcagctatgaacaagca (R) aaagttgggggagttctcgt | 303 |
mWnt3a | NM_009522 | (F) cccaacttctgcgaacctaa (R) tctccgccctcaagtaagaa | 347 |
mWnt4 | NM_009523 | (F) aacggaaccttgaggtgatg (R) ggacgtccacaaaggactgt | 345 |
mWnt5a | NM_009524 | (F) ctggcaggactttctcaagg (R) ctctagcgtccacgaactcc | 388 |
mWnt5b | NM_009525 | (F) gggaccgtttgaaggagaag (R) ctcttgaagcggtcatagcc | 259 |
mWnt6 | NM_009526 | (F) tcagttccagttccgtttcc (R) ttgacttctcatccccgaag | 323 |
mWnt7a | NM_009527 | (F) cgagagctaggctacgtgct (R) acagcacatgaggtcacagc | 264 |
mWnt7b | NM_009528 | (F) tacctaagttccgcgaggtg (R) acgtgttgcacttgacgaag | 363 |
mWnt8a | NM_009290 | (F) tgtcatggcatctcaggaag (R) gcggttgcagtagtcaggag | 240 |
mWnt8b | NM_011720 | (F) gtggacttcgaagcgctaac (R) ttacacgtgcgtttcatggt | 316 |
mWnt9a | NM_139298 | (F) tgctttcctctacgccatct (R) tatcaccttcacacccacga | 256 |
mWnt9b | NM_011719 | (F) aggagacggccttcctgtat (R) gcacttgcaggttgttctca | 296 |
mWnt10a | NM_009518 | (F) gcgctctgggtaaactgaag (R) cacacggttgttgtggagtc | 302 |
mWnt10b | NM_011718 | (F) ggaagggtagtggtgagcaa (R) cacttccgcttcaggttttc | 298 |
mWnt11 | NM_009519 | (F) gtgcggacaacctcagctac (R) gacaggtagcgggtcttgag | 259 |
mWnt16 | NM_053116 | (F) ctgtgaaaccaccttgcaga (R) caggttttcacagcacagga | 261 |