Table 3 Top 10% and 20% most significant time-dependent signaling metrics as identified via PLS
From: Data-driven modeling of cellular stimulation, signaling and output response in RAW 264.7 cells
Cytokine | Top 10% metrics | Top 20% metrics (not including those in top 10%) |
|---|---|---|
G-CSF | JNK lg: AUC, maximum | JNK lg: mean, @ 30 min, derivative @ 10 min, @ 10 min |
| Â | JNK sh: AUC | JNK sh: derivative @ 10 min, maximum, mean, @ 30 min, @ 10 min |
| Â | Â | P38: @ 30 min, @ 10 min |
| Â | Â | ERK1: derivative @ 10 min |
| Â | Â | ERK2: derivative @ 10 min |
| Â | Â | RSK: derivative @ 10 min, @ 30 min |
|  |  | NF-κB p65: @ 10 min, AUC, @ 30 min, maximum |
| Â | Â | PKCM: derivative @ 30 min |
IL-1α | JNK lg: maximum | JNK lg: AUC, @ 30 min, mean, derivative @ 10 min, @ 10 min |
| Â | JNK sh: AUC | JNK sh: maximum, mean, derivative @ 10 min, @ 30 min, @ 10 min |
| Â | Â | ERK1: derivative @ 10 min |
| Â | Â | ERK2: derivative @ 10 min |
| Â | Â | P38: @ 30 min |
| Â | Â | RSK: derivative @ 10 min |
| Â | Â | PKCM: derivative @ 30 min |
|  |  | NF-κB p65: @ 10 min, AUC, maximum, @ 30 min |
IL-6 | STAT3: mean, AUC, derivative @ 3 min, @ 3 min, maximum, @ 1 min, derivative @ 1 min, @ 10 min | STAT3: @ 30 min |
|  |  | STAT1α: derivative @ 10 min |
|  |  | STAT1β: derivative @ 10 min |
IL-10 | RSK: derivative @ 10 min | Â |
| Â | ERK2: derivative @ 10 min | Â |
| Â | ERK1: derivative @ 10 min | Â |
| Â | JNK sh: derivative @ 10 min, @ 30 min, AUC, @ 10 min | JNKsh: maximum, mean |
| Â | JNK lg: @ 30 min, @ 10 min, derivative @ 10 min | JNK lg: AUC, maximum, mean |
| Â | P38: derivative @ 10 min | P38: @ 30 min |
|  | NF-κB p65: AUC | NF-κB p65: maximum, @ 30 min, @ 10 min, mean |
| Â | Â | GSK3A: derivative @ 10 min |
MIP-1α | JNK lg: mean, maximum, AUC |  |
|  | NF-κB p65: @ 30 min, derivative @ 30 min | NF-κB p65: @ 10 min |
| Â | JNK sh: maximum, AUC | JNK sh: mean, derivative @ 10 min |
| Â | STAT5: 1 min, derivative @ 1 min | Â |
| Â | Â | ERK2: derivative @ 10 min |
| Â | Â | ERK1: derivative @ 10 min |
| Â | Â | PKCM: maximum, derivative @ 30 min |
| Â | Â | P38 @ 30 min, maximum, @ 10 min |
|  |  | STAT1α: derivative @ 30 min |
RANTES | JNK lg: maximum, AUC, mean | JNK lg: derivative @ 10 min, @ 30 min, @ 10 min |
| Â | JNK sh: maximum, AUC, derivative @ 10 min | JNK sh: mean, @ 10 min, @ 30 min |
| Â | ERK2: derivative @ 10 min | Â |
| Â | ERK1: derivative @ 10 min | Â |
| Â | Â | RSK: derivative @ 10 min |
| Â | Â | PKCM: derivative @ 30 min, maximum |
| Â | Â | P38: derivative @ 10 min, @ 30 min |
|  |  | NF-κB p65: @ 10 min |
TNFα | NF-κB p65: @ 30 min, @ 10 min |  |
| Â | JNK lg: mean, maximum, AUC | Â |
| Â | Â | PKCM: derivative @ 30 min, @ 30 min, maximum |
| Â | Â | P38: @ 30 min, @ 10 min, AUC |
| Â | Â | JNK sh: maximum, AUC, mean |
| Â | Â | RSK: @ 30 min |
| Â | Â | ERK1: @ 30 min |
| Â | Â | Rps6: derivative @ 30 min |
| Â | Â | ERK2: @ 30 min |