SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation

Table 1 Electrical resistance across monolayers of cultured Line 31E mammary epithelial cells

Cell Type	Treatment	Transmonolayer resistance* (Ohms × cm²)
31E-pBabeVector	w/o Wnt-3a	290 +/- 22
	+ Wnt-3a	620 +/- 36
31E-pBabeSFRP-4	w/o Wnt-3a	250 +/- 18
	+ Wnt-3a	310 +/- 27

* Cultures previously selected with puromycin were grown on nitrocellulose filter inserts (2.2 cm diameter) for 48 hrs prior to adding treatment with or without Wnt-3a at 5 μl/ml for a further 28 hrs. Values shown are the mean +/- S.E.M. of 5 measurements made on parallel inserts taken across the membrane from apical to basal sides with the Millicell ERS instrument.