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Figure 3 | Journal of Molecular Signaling

Figure 3

From: SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation

Figure 3

Wnt signalling induces phosphorylation and activation of Akt/PKB. (a) Upon stimulation with 5 μl/ml of purified Wnt-3a for 4 hours, Line 30F cells show an elevation of phoshorylated Akt in both Ser473 (panel 1) and Thr308 (panel 2) which coincides with phosphorylation of GSK3β at Ser9/21 (panel 4), reflecting its inactivation by Akt. Total Akt was used as a loading control by stripping the membrane and reprobing with an anti-Akt antibody (panel 3). β-actin was used as a loading control for pGSK-3β (panel 5). (b) Treatment of 31E + 30F co-cultures with purified Wnt-3a for 2 and 4 hours also resulted in phosphorylation of Akt at Ser473 (panel 1) and of GSK-3α and -3β at Ser9/21 (panel 3).

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