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Figure 2 | Journal of Molecular Signaling

Figure 2

From: SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation

Figure 2

Accumulation of β-catenin in the cytosol upon stimulation with Wnt-3a. Line 30F cells (a), and co-cultures of Lines 31E + 30F or C57MG cells (b) were stimulated with purified Wnt-3a or mock-purified conditioned medium (Control) for the indicated times. Free cytoplasmic β-catenin was obtained by hypotonic lysis using Dounce homogenization followed by immunoblot analysis with a broadly reactive polyclonal, anti-β-catenin antibody. Increased "free" cytosolic β-catenin is evident even after half an hour of Wnt-3a treatment of C57MG cells (b), and is sustained for up to the 6 hour time point examined with 30F cells (a). Immunodetection of β-actin served as a loading control.

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Journal of Molecular Signaling

ISSN: 1750-2187