Figure 7

Translocation of Bax and p53 to mitochondria. (A), Resveratrol induces translocation of Bax and p53 to the mitochondria. LNCaP cells were treated with or without resveratrol (20 μM) for 2 or 4 h. Cells were fixed, permeabilized and stained with anti-Bax antibody at 4°C for 18 h. After washing, cells were stained with mitotracker red (mitochondrial staining), DAPI (nuclear staining) and secondary antibody conjugated with FITC (for Bax). Red color = mitochondria, green color = Bax, blue color = nucleus, yellow = translocation of Bax or p53 to mitochondria. (B), Translocation of Bax and p53 to mitochondria. LNCaP cells were treated with resveratrol for 0, 2, 4 or 8 h. Mitochondrial fractions were prepared, subjected to SDS-PAGE, and immunoblotted with anti-Bax, anti-p53 or anti-COX IV antibody.